Impact Of Practices Research Articles

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antimicrobial resistance (AMR) in slurry. Temporal fluctuations in cephalosporin-resistant Escherichia coli were observed and attributed to farm activities, specifically the disposal of spent copper and zinc footbath into the slurry system. Our model revealed that resistance should be more frequently observed with relevant determinants encoded chromosomally rather than on plasmids, which was supported by reanalysis of sequenced genomes from the farm. Additionally, lower resistance levels were predicted in conditions with lower growth and higher death rates. The use of muck heap effluent for washing dirty channels did not explain the fluctuations in cephalosporin resistance. These results highlight farm-specific opportunities to reduce AMR pollution, beyond antibiotic use reduction, including careful disposal or recycling of waste antimicrobial metals.

Effectiveness of vestibular rehabilitation on postural balance in Parkinson’s disease: a systematic review and meta-analysis of randomized controlled trials

IntroductionPostural balance impairment can affect the quality of life of patients with Parkinson’s disease. Previous studies have described connections of the vestibular system with postural functions, suggesting a potential participation of the basal ganglia in receiving vestibular stimuli. This systematic review aims to summarize the evidence on the effectiveness of vestibular rehabilitation on postural balance in patients with Parkinson’s disease.MethodsA systematic review was conducted using the electronic databases: PubMed, Embase, Scopus and PEDro. The study selection was independently conducted by two reviewers, and disagreements were evaluated by a third reviewer. The included studies had no restrictions on publication dates or languages and the last update occurred in July 2023.ResultsFrom the 485 studies found in the searches, only 3 studies were deemed eligible for the systematic review involving a total of 130 participants. The Berg Balance Scale was described as the tool for evaluation of postural balance in all studies. The meta-analysis showed statistically significant results in favor of vestibular rehabilitation (MD = 5.35; 95% CI = 2.39, 8.31; P < 0.001), regardless of the stage of Parkinson’s disease. Although the effect size was suggested as a useful functional gain, the analysis was done with caution, as it only included 3 randomized controlled trials. The risk of bias using the RoB-2 was considered as being of “some concern” in all studies. Furthermore, the quality of the evidence based on the Grading of Recommendations Assessment Development and Evaluation system, produced by pooling the included studies was considered very low.ConclusionCompared to other interventions, vestibular rehabilitation has potential to assist the postural balance of patients with Parkinson’s disease. However, the very low quality of the evidence demonstrates uncertainty about the impact of this clinical practice. More robust studies are needed to confirm the benefits of this therapy in patients with Parkinson’s disease. This study was prospectively registered in PROSPERO: CRD42020210185.

DivFolio: a Shiny application for portfolio divestment in green finance wealth management

Abstract This paper introduces DivFolio, a multiperiod portfolio selection and analytic software application that incorporates automated and user-determined divestment practices accommodating Environmental Social Governance (ESG) and portfolio carbon footprint considerations. This freely available portfolio analytics software tool is written in R with a GUI interface developed as an R Shiny application for ease of user experience. Users can utilize this software to dynamically assess the performance of asset selections from global equity, exchange-traded funds, exchange-traded notes, and depositary receipts markets over multiple time periods. This assessment is based on the impact of ESG investment and fossil-fuel divestment practices on portfolio behavior in terms of risk, return, stability, diversification, and climate mitigation credentials of associated investment decisions. We highlight two applications of DivFolio. The first revolves around using sector scanning to divest from a specialized portfolio featuring constituents of the FTSE 100. The second, rooted in actuarial considerations, focuses on divestment strategies informed by environmental risk assessments for mixed pension portfolios in the US and UK.

The influence of undetected hemolysis on POCT potassium results in the emergency department.

This study aimed to evaluate discrepancies in potassium measurements between point-of-care testing (POCT) and central laboratory (CL) methods, focusing on the impact of hemolysis on these measurements and its impact in the clinical practice in the emergency department (ED). A retrospective analysis was conducted using data from three European university hospitals: Technische Universitat Munchen (Germany), Hospital Universitario La Paz (Spain), and Erasmus University Medical Center (The Netherlands). The study compared POCT potassium measurements in EDs with CL measurements. Data normalization was performed in categories for potassium levels (kalemia) and hemolysis. The severity of discrepancies between POCT and CL potassium measurements was assessed using the reference change value (RCV). The study identified significant discrepancies in potassium between POCT and CL methods. In comparing POCT normo- and mild hypokalemia against CL results, differences of-4.20 % and +4.88 % were noted respectively. The largest variance in the CL was a +4.14 % difference in the mild hyperkalemia category. Additionally, the RCV was calculated to quantify the severity of discrepancies between paired potassium measurements from POCT and CL methods. The overall hemolysis characteristics, as defined by the hemolysis gradient, showed considerable variation between the testing sites, significantly affecting the reliability of potassium measurements in POCT. The study highlighted the challenges in achieving consistent potassium measurement results between POCT and CL methods, particularly in the presence of hemolysis. It emphasised the need for integrated hemolysis detection systems in future blood gas analysis devices to minimise discrepancies and ensure accurate POCT results.

Impact of Lean Manufacturing Practices on Waiting Time of Patients in Outpatient Department (OPD): A Study in the Sri Lankan Public Healthcare Industry.

This study aims to explain the impact of lean manufacturing practices on waiting time of patients at the outpatient department (OPD) in the Public hospitals. Cross-sectional data using a survey sample of 384 patients in the Kandy public hospitals with a satisfied random sampling were used. Data were analyzed using SPSS software. This study covers the four independent variables of lean manufacturing practices namely: 5S, visual management, kaizen, and Just in Time. The dependent variable is waiting time. The measurement instrument for lean manufacturing practices and waiting time was developed from comprehensive literature reviews. Results indicate that there is a negative significant impact of lean manufacturing practices on waiting time of patients in outpatient department (Beta = -0.626 &amp; p=0.000). Therefore, these findings suggest that the theories of lean manufacturing and the knowledge-based view providing implementation of lean manufacturing practices can reduce waiting time for patients in outpatient department.

Ethical management and stakeholder relations: Insights from Slovak companies with foreign participation

The practice of ethical management has gained traction due to its role in enhancing stakeholder relations, which can have severe repercussions for organisations. By prioritising ethics, companies not only uphold moral principles but also gain a competitive advantage. This is particularly true in societies that value socially responsible business and give preference to companies that go beyond the requirements of the law. Understanding the significance of ethical management practices is therefore becoming key to creating a responsible and sustainable business environment that benefits both an organisation and its stakeholders, such as employees, consumers and society. The purpose of this article is to present a comprehensive exploration of the impact of selected aspects of ethical management in Slovak companies with foreign participation on the ethicality of their relationships with stakeholders. By examining a range of factors related to ethical management, the article seeks to identify statistically significant differences among companies with different approaches to managing business ethics. Employing this analysis, the article contributes to the understanding of ethical practices in Slovak companies and provides insights for academics and practitioners of business ethics. The data used for this analysis was collected through an online questionnaire survey, resulting in a sample size of 179 monitored subjects, all of whom are Slovak companies with foreign participation. The research design included two groups of factors: “general factors of business ethics” or “ethical management approaches” and “ethicality of company-stakeholder relationships.” The statistical analysis included the Shapiro-Wilk normality test, followed by the non-parametric Kruskal-Wallis H test, and post hoc analysis using the Bonferroni adjustment for previously identified significances. The results of the research presented in the article indicate a predominantly positive ethical stance towards employees, suppliers, customers and other stakeholders among Slovak companies. Statistically significant differences were found in the levels of ethicality in relation to legal form, with limited liability and joint-stock companies showing different perceptions towards supplier ethics. The research also proves that an ethical organisational climate is a major determinant of the ethicality of Slovak companies and suggests that a robust integration of ethics into strategic planning significantly improves their stakeholder relations. It can also be concluded that the scope of a code of ethics is particularly significant for community relations, whereas the frequency with which it is updated has less impact. This research holds significant value because it explores the impact of ethical management practices on stakeholder relations and ethical issues in Slovak companies with foreign participation. By focusing on the specific context of Slovak companies, the research offers unique insights into the relationship between ethical management factors and stakeholder dynamics. This research aims to bridge a gap by shedding light on the intricate dynamics between ethical management and stakeholder relations. The findings provide valuable guidance to organisational leaders, policymakers and stakeholders in fostering ethical behaviour and mitigating ethical risks within companies.

Analysis of the sustainability index for ecologically low-input integrated farming: A comprehensive assessment of environmental, economic, and social impact

A comprehensive sustainability index was designed in this study to evaluate the effectiveness and environmental impact of ecologically low-input integrated farming systems. By integrating diverse agricultural practices while minimizing reliance on external, non-renewable resources, this approach aims to enhance ecological harmony and sustainability. The index provides a multi-dimensional assessment, encompassing environmental, economic, and social aspects, to gauge the overall sustainability of these integrated farming practices. The procedure involved defining critical sustainability criteria across environmental, economic, and social dimensions, collecting relevant data on farming practices, and constructing a model to assess and score these practices. The index was validated through real-world applications and engaged with various stakeholders for insights and practical verification. Environmental data involves measuring soil nutrients (5 %), water usage (500 gallons/acre), and biodiversity (30 species/acre) to assess the ecological impact of farming practices. Economic data focuses on recording crop yields, input costs, and market prices to evaluate the financial viability of these practices. Social data is collected through surveys on labor practices, job satisfaction, and community impact, providing insights into the social implications and acceptability of the farming methods. The data revealed that the farming system aligned well with sustainability benchmarks, exhibiting no deviation from optimal levels in soil nitrogen content and water usage. Strong profitability and input cost efficiency were observed in the economic dimension, with a net income per acre of $400/acre and an input cost efficiency of 50 %. In the social aspect, although reasonably scored, specific labor metrics and regional/industry standards were lacking for a comprehensive evaluation. Overall, the farming system demonstrated commendable sustainability practices, with an environmental score of 7.5/10, an economic score of 8/10, and a social score of 7/10, suggesting potential for further improvements, particularly in addressing social sustainability factors.

The impact of sugammadex dosing and administration practices on potential cost savings for pharmacy departments.

In an effort to expedite the publication of articles, AJHP is posting manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time. Neuromuscular blocking agents (NMBAs) are commonly used during surgery, and restoring neuromuscular function at the end of surgery is vital in preventing complications of residual paralysis. Recent guidelines from the American Society of Anesthesiology recommend using sugammadex over neostigmine; however, sugammadex is significantly more expensive than neostigmine and may increase drug expenditure for pharmacy departments. This review summarizes evidence evaluating sugammadex dose adjustments and the potential of these dose adjustments to lead to cost savings for pharmacy departments. We found evidence suggesting that the manufacturer-recommended sugammadex dose may not be needed in many patients and that dosing based on an adjusted body weight or based on depth of neuromuscular blockade may lead to lower sugammadex usage. Combining sugammadex with neostigmine could also decrease the dose of sugammadex required. We have highlighted the importance of objective neuromuscular monitoring to guide sugammadex dosing and evaluation for residual neuromuscular blockade. Cost savings for pharmacy departments may be realized by these alternative dosing strategies and/or vial-splitting practices. Implementing practices related to sugammadex vial splitting and dose adjustment based on body weight and depth of neuromuscular blockade shows potential benefits in terms of sugammadex cost savings.

Indigenous and colonial influences on Amazonian forests

Societal Impact StatementGlobal climate models that incorporate carbon sources and sinks usually consider that forest uptake of carbon is in a state of equilibrium. Both historical and paleoecological records suggest that this is commonly not the case for Amazonia. Here, the impacts of colonial practices on Amazonian Indigenous peoples and forests are reviewed. Human activities affect forests' successional stages, trajectories, and species composition. By increasing the spatial coverage of paleoecological records that focus on pre‐ and post‐Columbian periods, the long‐term interactions between humans and Amazonian forests and their role in affecting Earth's climate may be better understood.SummaryLegacy effects left by the activities of Indigenous people in Amazonia are well known. Although severe, widespread, and recently occurring, the impacts left post‐1492 CE have been less investigated. We review the impact of colonial practices on Indigenous peoples and Amazonian forests. We suggest that forests comprise the sum of their past events, in a mosaic of different cumulative successional trajectories depending on the type, frequency, intensity, and timing of human influence. In regions with a history of minimal human influence, old‐growth species sensitive to fire would be the dominant landscape. In regions with high pre‐Columbian and low colonial influence, old‐growth forests carrying pre‐Columbian ecological legacies would be prevalent. Regions occupied by Indigenous groups post‐1492 CE would also carry similar ecological legacies. In regions influenced by the Jesuits, mid‐successional forests are expected to be enriched with cacao trees. In regions of latex extraction during the rubber boom, mid‐growth forests would present high abundances of early and mid‐successional species and depletion of some species. In deforested areas, we expect early successional forests with influence of exotic useful species. This patchwork of history probably plays a large role in shaping today's forests, and the biodiversity and carbon dynamics documented within them. Paleoecological work focusing on the last millennium, although scarce, has the potential to detect these mosaics of past human influence, and they should be considered when estimating forest ages and successional stages across the basin.

INFLUENCE OF HUMAN RESOURCE MANAGMENENT STRATEGIES IN CONSTRUCTION PROJECTS

In the dynamic landscape of project management understanding the significant relationship between human resource management functions and project health has become an essential aspect. This research has invested in a primary quantitative investigation focused on the INDIAN construction sector through which the relationship between human resource management functions and project health would be analysed and their contribution to enhancing project success rate would also be evaluated. With the growing emphasis on efficient resource utilisation and project Optimisation, the study would help into the impact of HRM practices on project teams. Scrutinisation of HRM Strategies and their Correlation with Project Success, this research has aimed at offering significant insights that can revolutionise HRM approaches within the construction sector that can ultimately enhance project health and ensure high success rates in INDIAN construction projects.

Impact of the Great Invocation Practice at Vellalar College of Education

This paper explores the integration and impact of The Great Invocation, a prayer adapted by Alice Bailey, within the context of Vellalar College of Education. Since its introduction in 2018 as part of Yoga Prana Vidya (YPV) practices, The Great Invocation has become a daily ritual at the college, fostering spiritual alignment and personal growth among students and staff. Through a diverse body of research, the paper examines the positive outcomes associated with holistic practices, such as stress reduction, improved academic performance, and enhanced well-being. The influence of The Great Invocation on student well-being, teacher-student relationships, campus dynamics, and institutional achievements is explored, highlighting its significance in contributing to a culture of holistic development and continuous improvement within educational settings.

Revisiting façade design and its contribution to the urban experience in the 21st century

ABSTRACT As one of the most exteriorized architectural elements, façades are significant features of the urban image and important contributors to the aesthetic and other urban experiences. However, discussions on the subject have lost relevance in academic and professional circles following arguments about physical determinism, façadism, beautification, and aestheticisation practices. Reflecting on the multiple contributions of façades to contemporary urban life, this article re-engages with the debate on façade design and the aesthetic and other urban experiences it affords from a political perspective to interrogate the impact of individualistic design practices in an era dominated by capitalist place-making.

DEVELOPMENT OF INNOVATIVE TECHNOLOGIES IN CONSTRUCTION AND CIVIL ENGINEERING IN THE CONTEXT OF DIGITALIZATION OF THE UKRAINIAN ECONOMY

Purpose. Construction and civil engineering, traditionally perceived as conservative industries, also feel the need to adapt to the demands of the time and introduce innovative technologies. Ukraine, entering the era of digital transformation, is actively developing and implementing new technologies in the construction industry. Therefore, systematization and coverage of key aspects of the impact of digital Transformation into the construction industry and civil engineering in Ukraine is the main task today. Methodology. The introduction of Internet of Things technologies in the construction industry expands the possibilities of building control and management. Findings. Sensors located in different parts of the structures allow you to monitor the condition of the building in real time, identify possible problems and ensure the safety of objects. Artificial intelligence is becoming an integral part of the digital transformation of construction. Machine learning algorithms help predict and optimize construction processes, which increases Efficiency of planning and resource management. The 3D printing system in construction is one of the promising and revolutionary innovations that is already influencing traditional approaches to construction processes. This digital technique will bring significant changes to the field of construction, accelerating and optimizing a number of stages from design to execution Originality. Analysis of current problems, especially now, in the difficult times of war, industrial construction in Ukraine, as well as around the world, is a promising direction for the development of other industries in general. Industrial construction is an important branch of the construction industry that specializes in the creation and operation of buildings and infrastructure facilities for industrial needs. Practical value. The introduction of innovative technologies in construction and civil engineering in the context of digitalization of the Ukrainian economy has a significant practical impact on various aspects of the industry: Improving Construction Productivity and Quality: Use of digital technologies such as the Internet of Things (IoT), artificial intelligence (AI), virtual reality (VR) and augmented reality (AR), allows you to increase the efficiency of construction processes, reduce the time of work and avoid errors; Cost optimization: Using machine learning algorithms to predict resource needs, such as materials and labor, allows you to rationalize costs and reduce construction costs.

The role of innovative human resource management practices, organizational support and knowledge worker effort in counteracting job burnout in the Polish business services sector.

This study focuses on analyzing the impact of innovative human resource management practices (IHRMP) on knowledge worker burnout, and how organizational support and employee effort help explain this relationship in the context of the business services sector. To explore the problem, investigated whether IHRMP have a significant negative impact on employee burnout, and organizational support and employee effort mediate the negative impact of IHRMP on employee burnout. A survey was conducted, collected using the computer assisted web interview method on 1000 knowledge workers employed at business services sector (BSS) organizations in Poland. The quantitative results obtained were analyzed using AMOS software to test the main statistical relationships and through structural equation modeling. The study outlines direct and indirect mechanisms to counteract perceived burnout among knowledge workers. The article contributes to the understanding of how IHRMP reduce burnout among knowledge workers and highlights the central importance of organizational support and employee effort as mediating factors against burnout in the context of high-skill, high-intensity work. The expected results in terms of application provide a proposal of measures for managers' consideration that can be implemented in the organization with a view to counteracting the incidence of burnout among BSS employees. Int J Occup Med Environ Health. 2024;37(2).

Tax Administration and Financial Performance of Manufacturing Firms in Kenya: A literature Review

This study explores the impact of tax administration practices on the financial performance of manufacturing firms in Kenya. The manufacturing sector is crucial for economic growth and job creation, making it essential to understand how tax policies affect its performance. Tax administration encompasses procedures and policies governing tax compliance, which have evolved over time. Effective tax administration is vital for economic stability and funding public services. However, inefficient practices can hinder firm productivity and growth. Drawing on tax planning theory and agency theory, this study examines how firms strategically manage tax liabilities and navigate principal-agent relationships to optimize financial performance. The empirical review highlights findings from previous studies on the relationship between tax administration practices and firm performance in Kenya. These studies underscore the importance of tax incentives, compliance costs, and governance mechanisms in shaping firm behavior and economic outcomes. By understanding the nuances of tax administration practices, policymakers and firms can implement strategies to enhance economic stability and growth in the manufacturing sector.

Human Resource Practice and Job Satisfaction in Private College of Kathmandu

Human resource practice includes conducting job analyses, planning personnel needs, recruiting the right people for the right job at the right time, performance appraisal, orienting and training, rewarding, new job opportunities, motivate them. This study is related to the impact of HR practices on employee job satisfaction. In private colleges of Kathmandu, employees are not satisfied with their jobs even in the presence of HR practices. For this study convenience, sampling technique is used and the limitations of our study are the private colleges in Kathmandu. A questionnaire has been used to collect primary data based on structured questions. The study's hypothesis was tested through correlation and regression analysis to inspect employee satisfaction. Findings investigate a significant relationship between HR Practice and job satisfaction. Moreover, salary, reward and recognition, career advancement, promotion, new job opportunities, participation in decision-making and working environment positively affect job satisfaction. Results show that HR practices (recruitment and selection, salary, training and development, new job opportunities, rewards, recognition, and motivation) have more significant effects on employees’ job satisfaction in comparison to the Working Environment. The empirical findings concluded that best HR Practices have a significant and positive effect on employee job satisfaction. Therefore, it is clear that employers should understand and implement the HR Practices correctly to take good work from their employees. Further research may be done to achieve a high level of job satisfaction.

The Impacts of HRM Practices on Employee Outcomes: The Mediating Role of HRM Effectiveness

Purpose: This study aimed to examine the impacts of human resource management (HRM) practices on employee outcomes (employee commitment, employee satisfaction, and employee turnover intention) through HRM effectiveness. Theoretical Framework: The literature recognized the importance of HRM effectiveness in achieving both organizational outcomes and employee outcomes. Numerous variables were examined as mediating variables between HRM practices and organizational as well as employee outcomes such as work attitudes, employee engagement, knowledge management, employee performance, and HRM effectiveness. Method: Collecting data using a questionnaire-based survey from a convenient sample of employees. Results and conclusion: The results pointed out a significant direct effect of HRM practices on employee job satisfaction and insignificant direct effects of HRM practices on employee commitment and employee turnover intention. Also, the results revealed that the impact of HRM practices on employee satisfaction is partially mediated by HRM effectiveness and the impacts of HRM practices on employee commitment and employee turnover intention are fully mediated by HRM effectiveness. Hence, it was concluded that employee outcomes are positively related to the extent to which HRM practices are effectively executed. Research implications: The organizations are required to expand their HRM practices bundle and appraise HRM practices in numerous areas including employee succession planning, talent development, employee empowerment, quality enhancement management, performance management, as well as merit-based training and promotion. Originality/value: This study puts emphasis on the role of HRM practices on employee outcomes such as employee commitment, employee job satisfaction, and employee turnover intention.

Operative management of primary hyperparathyroidism in Europe.

Multicentre studies have previously reported on national outcomes of surgery for primary hyperparathyroidism, but not investigated whether management and outcome are uniform among countries. This study investigated whether there are differences among European countries in operative management and outcome of surgery for primary hyperparathyroidism. Using data from Eurocrine®, a pan-European registry for endocrine surgeries, a retrospective observational cross-sectional multicentre study with 99 participating centres in 14 European countries was performed. Data on age, sex, calcium levels, operative strategy, conversion rate and rate of failed exploration were analysed for patients who underwent initial surgery for sporadic primary hyperparathyroidism. Primary outcome measures were intention to perform limited parathyroidectomy and the rate of hypercalcaemia at first follow-up. A total of 9548 patients were registered between 2015 and 2020. There were 7642 (80%, range 74.5-93.2%) females. There was intention to perform limited parathyroidectomy in 7320 of 9548 (76.7%) operations, ranging from 498 of 1007 (49.5%) to 40 of 41 (97.6%) among countries. Hypercalcaemia at first follow-up (median time to follow-up 15 days) was found in 416 of 9548 (4.4%) operations, ranging from 0 of 119 (0%) to 3 of 38 (7.9%) among countries. This study demonstrated large differences in the intention to perform limited parathyroidectomy for primary hyperparathyroidism among European countries, as well as differences in the rate of postoperative hypercalcaemia. Future studies are needed to evaluate the impact of these different healthcare practices on patient outcomes.

Unlocking Genetic Diversity and Germplasm Characterization with Molecular Markers: Strategies for Crop Improvement

Molecular markers have emerged as the most useful tools in assessment of genetic diversity and characterizing germplasm, for crop improvement. This review paper comprehensively analyzes the applications of molecular markers in diversity analysis and germplasm characterization. It underscores the significance of genetic diversity as the bedrock for plant breeding programs, enabling the development of improved varieties with desirable attributes such as higher yields, stress tolerance, and enhanced nutritional profiles. The paper provides an overview of various types of molecular markers, including hybridization-based markers (e.g., RFLPs) and PCR-based markers (e.g., RAPDs, AFLPs, SSRs, and SNPs). It discusses marker selection strategies, emphasizing the consideration of factors like polymorphism, informativeness, and the potential for multiplexing and high-throughput genotyping. Diversity analysis techniques, including principal component analysis (PCA), cluster analysis methods (UPGMA and Neighbor-Joining), and population structure analysis (model-based approaches like STRUCTURE), are detailed. These methods enable the assessment of genetic relationships, identification of subpopulations, and selection of diverse parents based on molecular marker data. The review further explores the applications of molecular markers in germplasm management, conservation, and utilization. It discusses the role of markers in targeted introgression of desirable traits from diverse sources, as well as the integration of genotypic and phenotypic data for association mapping, genomic prediction, and selection. Additionally, the paper addresses emerging technologies, such as next-generation sequencing (NGS) and the integration of molecular markers with other omics data. It also highlights the practical applications and impact of molecular markers in crop improvement programs, including marker-assisted selection (MAS), genomic selection, and the development of genetically modified crops. Finally, the review outlines challenges and future perspectives, including limitations0 of current technologies, the potential of emerging techniques like NGS, and the integration of molecular markers with other omics approaches for a comprehensive understanding of complex traits.

Impact of diversified cropping systems and fertilization strategies on soil microbial abundance and functional potentials for nitrogen cycling

Diversified cropping systems and fertilization strategies were proposed to enhance the abundance and diversity of the soil microbiome, thereby stabilizing their beneficial services for maintaining soil fertility and supporting plant growth. Here, we assessed across three different long-term field experiments in Europe (Netherlands, Belgium, Northern Germany) whether diversified cropping systems and fertilization strategies also affect their functional gene abundance. Soil DNA was analyzed by quantitative PCR for quantifying bacteria, archaea and fungi as well as functional genes related to nitrogen (N) transformations; including bacterial and archaeal nitrification (amoA-bac,arch), three steps of the denitrification process (nirK, nirS and nosZ-cladeI,II) and N2 assimilation (nifH), respectively. Crop diversification and fertilization strategies generally enhanced soil total carbon (C), N and microbial abundance, but with variation between sites. Overall effects of diversified cropping systems and fertilization strategies on functional genes were much stronger than on the abundance of bacteria, archaea and fungi. The legume-based cropping systems showed great potential not only in stimulating the growth of N-fixing microorganisms but also in boosting downstream functional potentials for N cycling. The sorghum-based intercropping system suppressed soil ammonia oxidizing prokaryotes. N fertilization reduced the abundance of nitrifiers and denitrifiers except for ammonia-oxidizing bacteria, while the application of the synthetic nitrification inhibitor DMPP combined with mineral N reduced growth of both ammonia-oxidizing bacteria and archaea. In conclusion, this study demonstrates a strong impact of diversified agricultural practices on the soil microbiome and their functional potentials mediating N transformations.